Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N-2

Hickey D. P. , LİM K., CAI R., Patterson A. R. , YUAN M., Sahin S. , ...Daha Fazla

CHEMICAL SCIENCE, cilt.9, sa.23, ss.5172-5177, 2018 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 9 Konu: 23
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1039/c8sc01638k
  • Dergi Adı: CHEMICAL SCIENCE
  • Sayfa Sayıları: ss.5172-5177

Özet

Enzymatic bioelectrocatalysis often requires an artificial redox mediator to observe significant electron transfer rates. The use of such mediators can add a substantial overpotential and obfuscate the protein's native kinetics, which limits the voltage of a biofuel cell and alters the analytical performance of biosensors. Herein, we describe a material for facilitating direct electrochemical communication with redox proteins based on a novel pyrene-modified linear poly(ethyleneimine). This method was applied for promoting direct bioelectrocatalytic reduction of O-2 by laccase and, by immobilizing the catalytic subunit of nitrogenase (MoFe protein), to demonstrate the ATP-independent direct electroenzymatic reduction of N-2 to NH3.