Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N-2
CHEMICAL SCIENCE, cilt.9, sa.23, ss.5172-5177, 2018 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 9 Sayı: 23
- Basım Tarihi: 2018
- Doi Numarası: 10.1039/c8sc01638k
- Dergi Adı: CHEMICAL SCIENCE
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.5172-5177
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Enzymatic bioelectrocatalysis often requires an artificial redox mediator to observe significant electron transfer rates. The use of such mediators can add a substantial overpotential and obfuscate the protein's native kinetics, which limits the voltage of a biofuel cell and alters the analytical performance of biosensors. Herein, we describe a material for facilitating direct electrochemical communication with redox proteins based on a novel pyrene-modified linear poly(ethyleneimine). This method was applied for promoting direct bioelectrocatalytic reduction of O-2 by laccase and, by immobilizing the catalytic subunit of nitrogenase (MoFe protein), to demonstrate the ATP-independent direct electroenzymatic reduction of N-2 to NH3.