Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N-2
CHEMICAL SCIENCE, vol.9, no.23, pp.5172-5177, 2018 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 9 Issue: 23
- Publication Date: 2018
- Doi Number: 10.1039/c8sc01638k
- Journal Name: CHEMICAL SCIENCE
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.5172-5177
- Süleyman Demirel University Affiliated: Yes
Abstract
Enzymatic bioelectrocatalysis often requires an artificial redox mediator to observe significant electron transfer rates. The use of such mediators can add a substantial overpotential and obfuscate the protein's native kinetics, which limits the voltage of a biofuel cell and alters the analytical performance of biosensors. Herein, we describe a material for facilitating direct electrochemical communication with redox proteins based on a novel pyrene-modified linear poly(ethyleneimine). This method was applied for promoting direct bioelectrocatalytic reduction of O-2 by laccase and, by immobilizing the catalytic subunit of nitrogenase (MoFe protein), to demonstrate the ATP-independent direct electroenzymatic reduction of N-2 to NH3.