Fertilization biology is one of the most important criteria used to identify as candidate genotype and variety. In this research pollens of 6 wild-grown blackberry shrubs, preselected from Isparta-Turkey, were used. In order to determine the pollen performance which is the basis of the studies on fertilization biology, the amount of pollen production, pollen viability degree and the morphological homogeneity rate were determined. In addition, temperature experiments (15(0)C, 20(0)C and 25(0)C) were performed to determine the optimum pollen germination condition. However, the effects of some growth regulators and mineral substances (GA(3), KNO3, BA) on pollen germination and tube growth were investigated. N6 had the highest values in terms of anther number (71.2) average number of pollens per anther (13151.7), number of pollen per flower (414245.7), and morphological homogeneity (95.8%). 20(0)C was found optimum temperature for pollen germination and tube growth. Gibberellic acid and Potassium nitrate were determined as promoter while benzyl adenine had inhibitory effect on pollen germination and tube growth. As a result of fertilization biology studies N6 can be thought promising, terms of pollen performance.