Objective: To reveal the effectiveness of amniotic membrane transplantation in corneal and conjunctival diseases. Material and Methods: The records of 76 patients (76 eyes) who underwent amniotic membrane transplantation using an overlay technique between January 2004 and October 2007 were evaluated retrospectively. The patients were divided into four groups: Group 1 (neurotrophic keratitis), Group 2 (microbial keratitis), Group 3 (bullous keratopathy) and Group 4 (symblepharon, conjunctival tumor, chemical burn, exposure keratopathy, band keratopathy, rheumatoid arthritis and vernal keratoconjunctivitis associated ulcer). The age, gender, change in visual acuity (according to Snellen chart), epithelization success, elapsed time for epithelization, and follow-up time were the outcome measures. Results: The mean age of the patients was 51.96 +/- 21.83 years (6-85 years, 67.2% male, 32.8% female). The mean follow-up time was 8.05 +/- 6.19 (3-27) months. The most frequent indication for amniotic membrane transplantation was neurotrophic keratitis (28.95%). The remaining 18.42% were microbial keratitis, 17.1% were symblepharon, 13.16% were bullous keratopathy and 22.37% were others. Epithelization was achieved in 80.2% of corneal diseases in 20.06 +/- 7.40 days. The elapsed time for epithelization (p=0.72) and epithelization success (p=0.14) was not significantly different between the groups. Visual acuity improved in 30 patients (51.7%), remained unchanged in 22 patients (37.9%), and decreased in 6 patients (10.4%). Visual acuities before and after the amniotic membrane transplantation were significantly different (p<0.001). Conclusion: Amniotic membrane transplantation is an effective treatment either alone or in combination with keratoplasty for various conjunctival and corneal diseases.