Pressures on water resources are increasing on a global scale. It is expected that there will be water scarcity in many countries, including our country, in the future. This is a concern for industries using water intensively, such as textiles. In order to ensure the sustainable use of water resources, it is necessary to know the water footprint values, especially in the sectors that use water intensively. In this study, water footprint values were investigated throughout the life cycle of cotton textile production. In this context, the water footprint values for (cotton production, ginning) cotton fabric production stage, transportation, consumer use and disposal at the end of life, and the total water footprint value for cotton fabric production throughout the life cycle were determined by literature research. The total water footprint values for cotton fabric production throughout its life cycle vary between 4,573 and 7,583 m3/ton. For the total water footprint value, 93-97% is associated with cotton production and 2-6% is from cotton fabric production. Total water footprint rate for ginning, transportation and consumer use was found to be 1% and the total water footprint value was found to be 55 m3/ton. Therefore, water footprint reduction for cotton fabric can be achieved by applying efficiency measures in cotton production, production processes and consumer use steps.