To evaluate the strengthening effect of five different fibers with different placement designs in root canal treated and intracoronally bleached premolars. Seventy extracted single-rooted premolars were distributed into 7 groups (G1-G7). Group 1 (G1) included the intact (I) teeth as the negative control. Group 2 (G2) included root canal treated, intra-coronally bleached and composite (C) restored teeth as the positive control. In the other five test groups after root canal treatment the teeth were intra-coronally bleached and fiber materials were placed into standard MOD cavities in the following different designs: an intracanal rigid fiber/Reforpost (G3,RF), an intracanal flexible fiber/ Everstick (G4, FF), four intracanal flexible pin fibers/Dentapreg Pin (G5,PF), an intercuspal flexible fiber /Dentapreg SFU (G6, IF) or an intra-coronal horseshoe-shaped/ Dentapreg SFU (G7,CF). All cavities were filled with a microfilled resin composite. Fracture resistance was tested using a universal testing machine under a crosshead speed of 1 mm/minute. One-way ANOVA and Duncan's Multiple Range tests were used for statistical analysis. Fracture types were recorded. The fracture resistance values in descending order were G1(I): 1190.97 N > G6 (IF): 1138.78 N > G5 (PF): 942.45 N > G3 (RF): 737.40 N > G4 (FF):694.29 N > G2 (C): 611.83 N > G7 (CF): 542.78 N. There were statistically significant differences among the groups (p <= 0.05). In all groups, repairable coronal oblique fractures were mostly observed. Flexible fibers placed intercuspally exhibited a significantly better strengthening effect than those of the intracanal flexible and rigid fibers (p < 0.05).