Walnut (Juglans regia L.) samples were collected during the 2000 and 2001 harvests from different selections of trees grown in Turkey. Important physical and biochemical properties of fruit were examined in these walnut types. At the end of this study, 10 types were selected and nut and kernel properties, fatty acid composition and the proximate composition of these selections which are commonly grown in Turkey were evaluated. Physical properties are attributed to the quality criteria of nuts. Investigated genotypes showed good quality according to the test results. The total oil content ranged from 61.97 to 70.92% while the crude protein ranged from 15.17 to 19.24%. Ash ranged from 1.26 to 2.06% while the moisture was found between 3.25-3.91% of the kernel. The total carbohydrate was calculated as between 8.05-13.23%. The oleic acid content of the oils ranged from 21.18 to 40.20% of the total fatty acids, while the linoleic acid content ranged from 43.94 to 60.12% and the linolenic contents from 6.91 to 11.52%. It was found that palmitic acid was between 5.24 and 7.62%, while stearic acid ranged from 2.56 to 3.67%.