Autophagy and mTOR pathways in mouse embryonic stem cell, lung cancer and somatic fibroblast cell lines

OLTULU F., Kocaturk D. C. , Adali Y., ÖZDİL BAY B., Acikgoz E., GÜREL Ç., ...More

JOURNAL OF CELLULAR BIOCHEMISTRY, vol.120, no.10, pp.18066-18076, 2019 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 120 Issue: 10
  • Publication Date: 2019
  • Doi Number: 10.1002/jcb.29110
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.18066-18076


Embryonic developmental stages and regulations have always been one of the most intriguing aspects of science. Since the cancer stem cell discovery, striking for cancer development and recurrence, embryonic stem cells and control mechanisms, as well as cancer cells and cancer stem cell control mechanisms become important research materials. It is necessary to reveal the similarities and differences between somatic and cancer cells which are formed of embryonic stem cells divisions and determinations. For this purpose, mouse embryonic stem cells (mESCs), mouse skin fibroblast cells (MSFs) and mouse lung squamous cancer cells (SqLCCs) were grown in vitro and the differences between these three cell lines signalling regulations of mechanistic target of rapamycin (mTOR) and autophagic pathways were demonstrated by immunofluorescence and real-time polymerase chain reaction. Expressional differences were clearly shown between embryonic, cancer and somatic cells that mESCs displayed higher expressional level of Atg10, Hdac1 and Cln3 which are related with autophagic regulation and Hsp4, Prkca, Rhoa and ribosomal S6 genes related with mTOR activity. LC3 and mTOR protein levels were lower in mESCs than MSFs. Thus, the mechanisms of embryonic stem cell regulation results in the formation of somatic tissues whereas that these cells may be the causative agents of cancer in any deterioration.