© 2020 Refik Saydam National Public Health Agency (RSNPHA).Objective: Methicillin-resistant Staphylococcus aureus (MRSA) is among the most prevalent pathogens causing healthcare-associated infections. Healthcare workers may act as reservoirs for the spread of MRSA to patients and other healthcare workers. The purpose of this study was to determine the prevalence of nasal carriage rate of S. aureus in hospital staff in Turkey. Methods: Meta-analysis by PRISMA guidelines was performed. We performed a systematic search of published studies in national and international databases from 1990 to 2019. Of the remaining 440 Articles, only 26 studies were included in this meta-analysis. The Cochrane Q statistic was calculated to assess the heterogeneity of results in studies. Heterogeneity among studies was evaluated using the I2 statistics. The effect size was estimated by reported with its 95% confidence interval. Results: Based on the 26 selected articles, the pooled prevalence of S. aureus and MRSA was 24.0% [95% confidence interval (CI): 0,19-0,29] and 16.0% (95% CI: 0,12-0.21), respectively. Heterogeneity between studies (I = 95,62, p=0.000 for S. aureus and I = 81,10 p =0.000 for MRSA) was found, so a random-effects model was used for the meta-analysis. This study is registered in the PROSPERO database (ID= CRD42018117306). Conclusion: Hospital infections are a significant problem for our country as it is all over the World. Healthcare workers may act as reservoirs for the spread of MRSA to patients and other healthcare workers. The most critical step in preventing hospital infection from hospital staff is training in hospital infection and hygiene. Cost-effective health policies should be developed in Turkey for the control and Prevention of hospital infections and resistant microorganisms.