Development of liquid chromatographic and UV-visible spectrophotometric methods for determination of pK(a) values of folic acid antimetabolites
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, cilt.212, 2022 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 212
- Basım Tarihi: 2022
- Doi Numarası: 10.1016/j.jpba.2022.114647
- Dergi Adı: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chimica, EMBASE, International Pharmaceutical Abstracts, MEDLINE, Veterinary Science Database
- Anahtar Kelimeler: Folic acid antimetabolites, pK(a) value, Acetonitrile-water mixtures, Acid-base equilibria, DISSOCIATION-CONSTANTS, IONIZABLE COMPOUNDS, CARBOXYLIC-ACIDS, RETENTION, SOLVATION, DRUGS, PREDICTION, RESOLUTION, MIXTURES
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
In this study, the values of ionization/protonation constants (pK(a)) of pemetrexed and raltitrexed, which are folic acid antimetabolites, were determined by reversed-phase liquid chromatography (RPLC) and UV-visible spectrophotometric methods at 25 degrees C. The (s)(s)pK(a) values were evaluated by retention time (t(R)) in binary acetonitrile-water mixtures with acetonitrile (ACN) contents of 13%, 15%, 17% and 20% (v/v) for the RPLC method and 13%, 15% and 17% (v/v) for the spectrophotometric method. The aqueous pK(a) values ((w)(w)pK(a)) of the studied compounds were calculated from the (s)(s)pK(a) value using the macroscopic parameters (mole fraction and dielectric constant), which play an important role in solvent properties. In addition, the degree of ionization of pemetrexed and raltitrexed was calculated using the (w)(w)pK(a) values.