Do Concentration or Photo-Activation Change Effect of Hydrogen Peroxide and Boric Acid on E.Faecalis Biofilm Hidrojen Peroksit ve Borik Asidin E. Faecalis Biyofilm Üzerindeki Etkisi Konsantrasyon veya Fotoaktivasyon ile Değişir Mi?


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Ahmet T., KÜÇÜK KELEŞ Ö., BAŞER KOLCU M. İ., Şababoğlu Baytaroğlu E., Belli S.

Selcuk Dental Journal, vol.13, no.1, pp.79-84, 2026 (Scopus, TRDizin)

  • Publication Type: Article / Article
  • Volume: 13 Issue: 1
  • Publication Date: 2026
  • Doi Number: 10.15311/selcukdentj.1744561
  • Journal Name: Selcuk Dental Journal
  • Journal Indexes: Scopus, Central & Eastern European Academic Source (CEEAS), Directory of Open Access Journals, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.79-84
  • Keywords: Biofilm, Boric acid, Germinator, Hydrogen peroxide
  • Open Archive Collection: AVESIS Open Access Collection
  • Süleyman Demirel University Affiliated: Yes

Abstract

Background: To evaluate the effects of hydrogen peroxide (H2O2) and boric-acid(BA) on E. faecalis biofilm with or without photo-activation by Germinator (Design for Vision, USA). Materials: Biofilm formation was provided on sterilized dentin discs. Five groups were created(n=8): 1.5%, 3% and 5% H2O2; 5 and 10% BA. Each was exposed to irrigation-solutions with or without photo-activation. ‘Biofilm killing-percentage’ (100%), (CFU/ml) was calculated, colony-counting was performed. One-way analysis of variance, Duncan, Tukey's tests were provided. Results: All irrigants reduced the number of viable-bacteria in biofilm (p<0.05). The results demonstrated that H2O2 was able to achieve 100% eradication of E. faecalis biofilm both with and without photoactivation. 10% BA with or without photo-activation was found more effective than 5% BA concentration (p<0.001). Effects of H2O2 with or without photo-activation on biofilm was higher than BA groups (p<0.001). Conclusions: H2O2 with or without photo-activation is an effective solution against E. faecalis biofilm. 5 and 10% BA seem promising against E. faecalis while 10% seems more effective regardless the effect of photo-activation.