A large number of viruses cause economically important diseases on tomato plants worldwide. The viruses causing serious damage to tomatoes in the greenhouses of West Mediterranean Region in Turkey were identified. For this purpose, three viruses were investigated. A total of 186 tomato leaf samples were collected from 6 locations. Leaf samples were serologically tested by DAS-ELISA technique for 3 viruses: Tomato spotted wilt virus (TSWV), Tomato yellow leaf curl virus (TYLCV) and Pepino mosaic virus (PepMV). It was found that 63.44% (118 samples) of the tested samples were infected with one or more viruses. ELISA tests showed that among 186 samples, 53 were infected with TSWV (28.48%), 43 were TYLCV (23.12%) and 22 were infected with PepMV (11.83%). Various combinations of mixed infections were also observed in different samples. DAS-ELISA positive leaf samples were further confirmed through mechanical transmission of pathogen to herbaceous indicator plants.