Manganese superoxide dismutase, glutathione peroxidase and catalase gene polymorphisms and clinical outcomes in acute kidney injury


Kidir V., Uz E. , Yigit A., ALTUNTAŞ A. , Yigit B., Inal S., ...More

RENAL FAILURE, vol.38, no.3, pp.372-377, 2016 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 3
  • Publication Date: 2016
  • Doi Number: 10.3109/0886022x.2015.1135468
  • Title of Journal : RENAL FAILURE
  • Page Numbers: pp.372-377
  • Keywords: Acute kidney injury, catalase, gene polymorphism, glutathione peroxidase, manganese superoxide dismutase, ACUTE-RENAL-FAILURE, DIABETIC CARDIOVASCULAR-DISEASE, OXIDATIVE STRESS, NEPHROPATHY, ASSOCIATION, PROTECTS, MNSOD, RISK, AKI

Abstract

Introduction The aim of this study was to evaluate the potential association of single gene polymorphisms of manganese superoxide dismutase (MnSOD), glutathione peroxidase 1 (GPX1) and catalase (CAT) with clinical outcomes of acute kidney injury (AKI). Materials and methods Ninety AKI patients and 101 healthy volunteers were included in the study. Determination of MnSOD rs4880, GPX1 rs1050450 and CAT rs769217 polymorphisms was performed using real-time polymerase chain reaction amplification. The duration of hospitalization of AKI patients, dialysis and intensive care requirements, sepsis, oliguria and in-hospital mortality rates were assessed. Results The MnSOD, GPX1 and CAT genotypes and allele frequencies of AKI patients did not differ significantly from those of healthy controls. In patients with a T allele in the ninth exon of the CAT gene, intensive care requirements were greater than those of patients with the CC genotype (p=0.04). In addition, sepsis and in-hospital mortality were observed significantly more frequently in patients with a T allele in the ninth exon of the CAT gene (p=0.03). Logistic regression analysis determined that bearing a T allele was the primary determinant of intensive care requirements and in-hospital mortality, independent of patient age, gender, presence of diabetes and dialysis requirements (OR 6.10, 95% CI 1.34-27.81, p=0.02 and OR 10.25, 95% CI 1.13-92.80, p=0.04, respectively). Conclusion Among AKI patients in the Turkish population, hospital morbidity and mortality were found to be more frequent in patients bearing a T allele of the rs769217 polymorphism of the CAT gene.