Conformatıon of apoptotic effect of the Viscum Album L. extracts on human breast cancer MCF-7 cells with TUNEL assay


Sakallı Çetin E., Bayram D., Kara M., Özgöçmen M., Candan İ. A.

5th International Congresson Cell Membranes and Oxidative Stress:Focus on Calcium Signalingand TRP Channels, Isparta, Turkey, 9 - 12 September 2014, vol.6, pp.408

  • Publication Type: Conference Paper / Summary Text
  • Volume: 6
  • City: Isparta
  • Country: Turkey
  • Page Numbers: pp.408

Abstract

Aim Mistletoe ( Viscum album L., VA) is a semiparasitic plant that grows on various trees and has a variety of biological compounds lectins, viscotoxins, alkaloids, triterpenes, and oligo- and polysaccharides with antitumoral (cytotoxic, antiangiogenic) as well as immunomodulating properties. Besides, it has a significant antitumor activity including inhibitory effects on tumor formation, growth, invasion, metastasis and antiangiogenesis. This work aimed to confirme the apoptotic effects of the mistletoe extracts Helixor A (HA), Helixor P (HP) and Helixor M (HM) on human breast cancer cell line MCF-7 using Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Materials and Methods MCF-7 cell line was cultured in multicellular spheroid model. Cells were treated with the mistletoe extracts HA, HM and HP on 24, 48 and 72 hours incubation. Cells were not treated with the mistletoe extracts were considered as the control group. Tunel staining were assessed by immunohistochemically. Results IC50 values of HP in MCF-7 are 500μg/ml, 50μg/ ml and 50μg/ml on 24, 48 and 72 hours incubation respectively. IC50 values of HA and HM in MCF-7 are 500μg/ml on all incubations. Tunel assay was used to determine the death of the cells. TUNEL positive cells were detected after treatment in multicellular spheroid model. Dead cell count was more in the mistletoe extracts HA, HM and HP applied MCF-7 cell lines in comparison to the controls (p <0.05). Conclusion Spheroids are known to be in vivo-like tissueculture representatives and the most adapted model to keep the in vitro resistance properties of cells. Due to these characteristics, spheroids quite realistically represent the results of the drug effects by including limitations in penetration, distribution, and feedback mechanisms in cell signaling In this study, mistletoe extracts HA, HM and HP applications enhanced the TUNEL positive cells in comparison to the controls in multicellular spheroid model.