Conformatıon of apoptotic effect of the Viscum Album L. extracts on human breast cancer MCF-7 cells with TUNEL assay
5th International Congresson Cell Membranes and Oxidative Stress:Focus on Calcium Signalingand TRP Channels, Isparta, Türkiye, 9 - 12 Eylül 2014, cilt.6, ss.408, (Özet Bildiri)
- Yayın Türü: Bildiri / Özet Bildiri
- Cilt numarası: 6
- Basıldığı Şehir: Isparta
- Basıldığı Ülke: Türkiye
- Sayfa Sayıları: ss.408
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Aim
Mistletoe ( Viscum album L., VA) is a semiparasitic plant that grows on various trees and has a
variety of biological compounds lectins, viscotoxins,
alkaloids, triterpenes, and oligo- and polysaccharides
with antitumoral (cytotoxic, antiangiogenic) as well
as immunomodulating properties. Besides, it has a
significant antitumor activity including inhibitory
effects on tumor formation, growth, invasion,
metastasis and antiangiogenesis.
This work aimed to confirme the apoptotic effects
of the mistletoe extracts Helixor A (HA), Helixor P
(HP) and Helixor M (HM) on human breast cancer
cell line MCF-7 using Terminal deoxynucleotidyl
transferase dUTP nick end labeling (TUNEL) assay.
Materials and Methods
MCF-7 cell line was cultured in multicellular
spheroid model. Cells were treated with the
mistletoe extracts HA, HM and HP on 24, 48 and
72 hours incubation. Cells were not treated with
the mistletoe extracts were considered as the
control group. Tunel staining were assessed by
immunohistochemically.
Results
IC50 values of HP in MCF-7 are 500μg/ml, 50μg/
ml and 50μg/ml on 24, 48 and 72 hours incubation
respectively. IC50 values of HA and HM in MCF-7 are
500μg/ml on all incubations. Tunel assay was used
to determine the death of the cells. TUNEL positive
cells were detected after treatment in multicellular
spheroid model. Dead cell count was more in the
mistletoe extracts HA, HM and HP applied MCF-7
cell lines in comparison to the controls (p <0.05).
Conclusion
Spheroids are known to be in vivo-like tissueculture representatives and the most adapted
model to keep the in vitro resistance properties of
cells. Due to these characteristics, spheroids quite
realistically represent the results of the drug effects
by including limitations in penetration, distribution,
and feedback mechanisms in cell signaling In this
study, mistletoe extracts HA, HM and HP applications
enhanced the TUNEL positive cells in comparison to
the controls in multicellular spheroid model.