The aim of this study is to investigate the acute effect of sprint swimming performance on elite male triathletes on lactate, glucose, Spo2 and pulse values. 12 male tohm triathlon athletes between the ages of 17-19 from Antalya province participated in this study. Triathletes' blood lactic acid measurements was taken by using lactateplus, glucose measurements were taken via accutrend glucometer, oxygen saturation measurements were accessed by implementing pulse oximeter, pulse values were reached by using the nimo brand pulse meter device three times including before the test (BT), after the test (AT) and 5 minutes after the test (5MinAT). The evaluation of the data was carried out via a statistical program in computer environment. In statistical analysis, minimum and maximum value, arithmetic mean and standard deviation values were calculated. The data obtained in terms of the features discussed in the study were analyzed by repeated measurements analysis technique in factorial order. In the study, "Bonferroni Test", which is one of the multiple comparison methods, was used to determine the differences between the level averages of the groups. The data were evaluated at "p <0.05" significance level. In our study, repeated measurements of elite triathletes were BT 1.96 +/-, 95 mmol / L, AT 9.5 +/- 2.22 mmol / L and 5MinAT 8.38 +/- 2.33 mmol / L, glucose repeat measurements BT 103.25 +/- 13.30 mg / dl, AT 114.68 +/- 20.88 mg / dl and 5MinAT 120.25 +/- 16.57 mg / dl, repeated measurements of heart rates were found to be BT 67.87 +/- 4.86 beats / min, AT 169.50 +/- 8.04 beats / min and 5MinAT 95.81 +/- 13.45 beats / min. While as a result of the analysis of repeated measures of La, glucose and heartbeat counts were found to have statistically significant differences (p <0.05), repeated measures of sp02 values of athletes were determined as BT 98,37 +/-, 80%, AT 98,37 +/-, 80% and 5Min AT 9843 +/-, 51%, and no statistical difference was found (p> 0.05). Consequently, we think, the severity of exercise and due to the exercise severity, emptying of the glycogen stores in the muscles, at the same time because the number of heart beats will increase depending on this situation, the decrease in 5MinAT measurements is due to the fact that the athletes quickly replace the muscle glycogen stores consumed during the test after the sprint swimming performance as the reason for the increase of the BT and AT values of triathletes. We anticipate that this study will be a resource for athletes and coaches in the future.