In this study, physico-chemical properties (total soluble solid contents, pH, titratable acidity, vitamin C, antioxidant activity, total phenolic and total anthocyanins) of black mulberry (Morus nigra L.) fruits grown in the Bitlis province of Turkey were investigated. The total soluble solids content of black mulberry varies between 15.65 % (13-BIT-2) and 22.1 % (13-BIT-6), titratable acidity between 1.45 % (13-BIT-1) and 1.85 % (13-BIT-4), pH between 3.65 (13-BIT-2) and 4.12 (13-BIT-5), respectively. Ascorbic acid (vitamin C) was in the range from 18.40 (13-BIT-3) to 23.67 (13-BIT6) mg/100 g fresh weight (FW). The highest total phenolic contents were found 1920 (13-BIT-2) to 2575 (13-BIT-7) mg of gallic acid equivalent (GAE) 100 g(-1) fresh weight. Antioxidant capacity (DPPH) was in the range from 18.24 (13BIT- 1) to 23.18 (13-BIT-6) % and total anthocyanin content between 643 (13-BIT-4) and 826 (13-BIT-8) mg/100 g.