This reasearch evaluated the intraoperative hemostatic effects of a novel hemostatic agent (Ankaferd Blood Stopper (ABS)) during periodontal surgery on antithrombotic condition, without interruption or diminution of the medication. In total, 20 rats which are on antithrombotic condition were randomized into 2 groups, each consisted of 10 rats. In group I (control group), local hemostasis was achieved with direct packing with gauze. In group II, local hemostasis was achieved by the local application of ABS on the site. The bleeding time was compared between 2 groups following the periodontal surgery. The bleeding time in the operation site applied with ABS was statistically lower compared to the control group. It is concluded that the periodontal surgical operations could be performed appropriately with ABS without interruption of the medication in rats on anthithrombotic condition.