Isolation and characterization of glutathione s-transferase and glutathione reductase in Kabarla strawberry variety


Nur C. G. , Damla G., Yasar K.

RESEARCH JOURNAL OF BIOTECHNOLOGY, cilt.16, sa.4, ss.75-80, 2021 (ESCI İndekslerine Giren Dergi) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Konu: 4
  • Basım Tarihi: 2021
  • Dergi Adı: RESEARCH JOURNAL OF BIOTECHNOLOGY
  • Sayfa Sayıları: ss.75-80

Özet

Reactive oxygen species (ROS) increase in plants due to abiotic and biotic stresses. ROS interferes with intracellular substances and cell membrane to damaged plants. Plants have enzymatic and non-enzymatic antioxidant systems to protect themselves against ROS. Glutathione reductase (GR) and glutathione s-transferase (GST) are two of the antioxidant enzymes. The sequence information of these enzymes, which are extremely important for coping with stress, has not been investigated in many plants including strawberries. In this study, GR and GST antioxidant enzyme genes were isolated and characterized from Kabarla strawberry variety. For this purpose, degenerated primers were designed and partial cDNAs were amplified by PCR, and then 3' and 5' RACE analysis were performed to obtain their full length nucleotide sequences.