The aim of the research was to determine the forage yield and quality of thirty alfalfa (Medicago sativa L.) genotypes. Plots were established in 2014 in Isparta, Turkey, in a randomized complete block design with 3 replicates. Five cuttings were done during the 2015, 2016 and 2017. The dry matter yield (DMY), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL) and relative feed value (RFV) were determined. According to the three years average results, significant differences were determined for all parameters. Alfalfa genotypes viz., Cay-1, Cay-2, Keciborlu-1 and Keciborlu-3 were selected for alfalfa breeding program due to their superior yield and quality features.