A folic Acid-Functionalized polydopamine nanobowl shaped nanomotors for the detection of miRNA-21
Microchemical Journal, cilt.212, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 212
- Basım Tarihi: 2025
- Doi Numarası: 10.1016/j.microc.2025.113521
- Dergi Adı: Microchemical Journal
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Food Science & Technology Abstracts, Index Islamicus, Veterinary Science Database
- Anahtar Kelimeler: Biomarker, Cancer cell, miRNA-21, NIR-driven nanomotor, PDA Nanobowl
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Thanks to the development of nanotechnology, diagnostic methods for diseases have progressed beyond traditional approaches, leading to the creation of biomarker-targeted diagnostic techniques. For breast cancer, which has certain diagnostic limitations, miRNAs have become highly regarded as diagnostic tools. In this study, we report the development of near-infrared (NIR) light-driven folic acid (FA) functionalized bowl-like polydopamine nanobowl (PDA NB) structures targeting miRNA-21, a specific biomarker for breast cancer. FA, which is preferentially absorbed by malignant tumor tissues, was chosen for modifying the bowl-like PDA NB structures to facilitate the diagnosis of target miRNA-21. The surface modification with FA improved the targeting and penetration capabilities of the PDA NB structures towards tumor tissue. The diagnostic efficacies of the PDA-FA-ssDNA NB structure in synthetic environments containing various concentrations of miRNA-21 were analyzed using three different methods: speed, fluorescence intensity (FI), and cyclic voltammetry (CV). The limit of detection (LoD) of PDA-ssDNA and PDA-FA-ssDNA were compared for each method. Analyses were performed at 12 different concentrations of the target, ranging from 0.005 nM to 100 nM. The LoD values were calculated according to speed, FI, and CVox and CVred were: LoDspeed = 0.23 nM and LoDFI = 0.35 nM, LoDox = 0.40 nM, and LoDred = 0.36 nM for PDA-ssDNA and PDA-FA-ssDNA respectively. The proximity of the LoD values to each other indicates good accuracy for the study. Following the diagnostic studies conducted in synthetic environments, in vitro studies supported by fluorescence microscopy were performed on MCF-7 cancer cell lines and MCF-10A healthy cell lines. The LoD value calculated from the in vitro analysis conducted on the MCF-7 cancer cell line was found to be 0.565 nM.