Wheat x Maize Crosses for Haploid Embryo Production and Comparison of haploid-diploid Embryo Structure


Arzu O., Cigdem S.

RESEARCH JOURNAL OF BIOTECHNOLOGY, vol.9, no.10, pp.32-37, 2014 (Journal Indexed in SCI) identifier

  • Publication Type: Article / Article
  • Volume: 9 Issue: 10
  • Publication Date: 2014
  • Title of Journal : RESEARCH JOURNAL OF BIOTECHNOLOGY
  • Page Numbers: pp.32-37

Abstract

The aim of this study was to compare haploid and diploid embryo structure derived from two different wheat varieties, Triticum aestivum L. (Gun 91) and Triticum durum Desf. (Mirzabey). For diploid embryos, plants were grown until earing time, dicamba and 2,4-D were also applied to spikes in flowering period and embryos were collected after 18th-21st days of following pollination. For haploid plants, embryo rescue technique was used to derive haploid embryos by using intergenic crosses between both wheat varieties and corn (Zea mays L.). Synthetic auxins were also used to support of haploid embryo production. Two different microscopic measurement, width and length, were taken in each of embryo obtained. The decreasing in width of embryo in dicamba treatment group and also in length of embryo in 2,4-D treatment group were found to be different significantly (p<0.05 and p<0.01) in Gun 91 wheat variety.