Hymenoptera diversity was investigated in Golcuk Natural Park, near Isparta, Turkey during 2008 vegetation period. Twenty one families were recorded during our study. Four species are new records from Turkey. This paper compares Hymenopteran communities in four sampling sites from Golcuk Natural Park. The highest Hymenoptera family level diversity was found near the Natural Park entrance, where the Shannon-Weaver index of diversity was 2.5. Hymenopterans were most abundant in the site close to the old apple orchard, where 33% of all individuals were collected. Most of less frequently recorded families were found in the xeric natural plant site. Hymenopteran families were found to be almost equally partitioned in all four sampling sites. The highest percentage similarity index (0.79) was found between the "close to entrance" and "mesophilic" plant sites. The study revealed that the site close to the old apple orchard provides a special micro-habitat for Hymenoptera.