Pratylenchus thornei, P. neglectus, P. penetrans and P. crenatus are common distributed Root lesion nematodes of temperate fruit orchards in the West Mediterranean region of Turkey. The objective of this study was to investigate host reaction of these root lesion nematodes on commonly used apple, cherry, peach and pear rootstocks in Turkey. Experiments were established on randomized block desing in screen house with 5 replica. Pratylenchus penetrans developed well and had high population density on the M-9, MM-106 and MM-111 apple rootstocks in year 1. Reproduction factor (Rf) of P. penetrans on M-9, MM-106 and MM-111 in the first year were 6,2, 3,0 and 12,9, respectively. In the second year, Rf of P. penetrans on M-9, MM-106 and MM-111 were 5,6, 2,5 and 10,6, respectively. OHF-333 and Quince-A pear rootstocks showed moderate resistant reaction to P. penetrans. Rf value of P. penetrans was found 1,0 on OHF 333 and 0,5 on Quince-A rootstocks in the first year. And also, similar results were observed on pear rootstocks in the second year. Peach rootstocks GF- 677 showed moderate susceptible reaction to P. penetrans in both two year. Nemaguard, GN (Garnem) and Myrabolon 29 C rootstocks had resistant to P. penetrans. Similarly, P. penetrans did not reproduce on Cherry rootstocks SL-64, Maxnna-14 and Gisela-5 in years 1 and 2. Pratylenchus crenatus, P. thomei and P. neglectus did not develope and Rf value of these lesion nematodes were determined lower than 1 value on apple, cherry, peach and pear rootstocks in the experiments.