Comparison of Three Analytical Methods to Determine the Amount of Thymoquinone in Black Cumin Seed Oil (Nigella sativa Linn)
7TH INTERNATIONAL CONFERENCES ON SCIENCE AND TECHNOLOGY , Durres, Arnavutluk, 4 - 06 Eylül 2024, ss.82-87, (Tam Metin Bildiri)
- Yayın Türü: Bildiri / Tam Metin Bildiri
- Basıldığı Şehir: Durres
- Basıldığı Ülke: Arnavutluk
- Sayfa Sayıları: ss.82-87
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Thymoquinone is a natural chemical found in the plant Nigella sativa. It can also be found in some cultivated Monarda fistulosa plants, which can be steam distilled to produce an essential oil. It has been classified as a pan-assay interference compound, which binds to many proteins indiscriminately. It is currently being researched to identify its possible biological properties (Enoli and Hugh, 2018). In this study, essential oil was obtained from Nigella sativa by distillation of water vapour. The amount of thymoquinone in the essential oil was analyzed by three different analytical methods using a high purity reference analytical standard of thymoquinone in order to compare the methods. In the present study, three chromatographic methods based on direct n-hexane extraction were validated for the thymoquinone quantification in Black Cumin Seed Oil samples, the first one by high-performance liquid chromatography combined with tandem mass spectrometry (UHPLC-MS/MS), the second one is by gas chromatography mass spectrometry (GC-MSD) and the third one is by gas chromatography flame ionization detector (GCFID). All analytical methods showed good linearity in the calibration range (R² > 0.99). The recoveries of thymoquinone were greater than 95 % with three techniques. The methods showed good precision, repeatability, and reproducibility. The LOD and LOQ values achieved for UHPLC-MS/MS were 0,299 and 0,996 μg/mL, for GC-MSD were 0,348 and 1.161 μg/mL, while for GC-FID were 0,456 and 1,519 μg/mL. The results demonstrate that all three methods exhibit high selectivity for the determination of thymoquinone. It can be concluded that all three methods are suitable for the determination of thymoquinone content in black cumin oil, although analyses by UHPLC-MS/MS are one step ahead.