This study was carried on to determine the effect of different irrigation regime on winter wheat crop and water-use efficiency (WUE). For this purposes the experiment was conducted with 4 different irrigation treatment which was I-1; Rainfed, I-2; Full irrigation (irrigate when calculated soil water depletion is 60 mm) I-3; Limited irrigation (2 irrigation maximum) one at tillering and another at grain filling, I-4; No irrigation after establishment until heading, after which irrigation when soil water depletion is 60 mm below field capacity at Saraykoy Research Station in Murted Basin. The experimental design was completely randomized block design with four replications. Soil moisture was measured with neutron probe. At the end of the research study conducted during the wheat growth period for the years 2009-2010 and 2010-2011, in average wheat yield was found to be 3.35 t ha(-1), 4.54 t ha(-1), 4.22 t ha(-1) and 4.31 t ha(-1) respectively according to the plots (I-1, I-2, I-3, I-4). The highest yield was obtained from the full-irrigation plot while the lowest yield was obtained from the no-irrigation plot. No statistically significant difference was found between the plots subjected to the irrigation treatments while a difference of P < 0.05 was obtained between the no-irrigation and full-irrigation plots. Average harvest index values were found to be respectively 29%, 31%, 32%, 31% and 32% again according to the plots. A significant negative correlation was found between grain yield, total harvested biomass and the WUE. The results presented in this work suggest that the amount of soil water content affects grain yield and water use efficiency. It might be recommended that irrigation concentrated in the after heading period increase WUE in Central Anatolia Region of Turkey. Crop water stress index is a useful tool for detecting crop water stress.