Optimization strategy for isocratic separation of alpha-aspartame and its breakdown products by reversed phase liquid chromatography

Demiralay E., Ozkan G.

CHROMATOGRAPHIA, vol.60, pp.579-582, 2004 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 60
  • Publication Date: 2004
  • Doi Number: 10.1365/s10337-004-0421-7
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.579-582
  • Süleyman Demirel University Affiliated: No


alpha-Aspartame (L-alpha-aspartyl-L-phenylalanine methyl ester) is increasingly being used in wide variety of commonly consumed food products. This structure contains ester and peptide bonds. The ester linkage may hydrolyze to produce L-alpha-aspartyl-L-phenylalanine or cyclohydrolyze to produce the corresponding cliketopiperazine ring. This ring can open to form L-alpha-aspartyl-Lphenylalanine and ultimately this compound can hydrolyze to L-phenylalanine and L-aspartic acid. The pH, buffer type, concentration, the presence of water and temperature are other important factors affecting its stability. Because a-aspartame can lose its sweetness under these conditions determination of aspartame and its breakdown products is extremely important for diet foods quality.