Melatonin increases NMDA receptor subunits 2A and 2B concentrations in rat hippocampus

Sutcu R., Yonden Z., Yilmaz A., Delibas N.

MOLECULAR AND CELLULAR BIOCHEMISTRY, vol.283, pp.101-105, 2006 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 283
  • Publication Date: 2006
  • Doi Number: 10.1007/s11010-006-2385-4
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.101-105
  • Süleyman Demirel University Affiliated: No


We investigated that the effects of melatonin on N-methyl-D-aspartat receptor (NMDAR) subunits 2A and 2B concentrations and on markers of oxidative stress in hippocampus of rats treated with melatonin for 4 weeks. Animals were divided into four groups according to their melatonin doses (control group (C), n = 7; 5 mg/kg group (M5), n = 7; 10 mg/kg group (M10), n = 8; 20 mg/kg group (M20), n = 8) and received the melatonin as intraperitoneal injection. After 4 weeks of treatment, electrophoretic examination was done using sodium dodecyl sulfate/polyacrilamide gel electrophoresis (SDS-PAGE) and Western blotting in hippocampal homogenates of different groups. Malondialdehyde (MDA), an end product of lipid peroxidation, was assayed in all groups. There were significant increases in both NMDAR 2A; 5 mg/kg group (n = 3, p < 0.05), 10 mg/kg group (n = 3, p < 0.05), 20 mg/kg group (n = 3, p < 0.05) and NMDAR 2B expressions; 5 mg group (n = 3, p < 0.05), 10 mg group (n = 3, p < 0.05), 20 mg group (n = 3, p < 0.05), compared to the control group. No significant difference in MDA concentrations was found between groups. The present study clearly demonstrates that melatonin alters hippocampal NMDAR subunit concentrations in a dose-dependent manner without causing lipid peroxidation.