Purification of Naturally Found Carvacrol Compound in Oregano Thyme by Flash Chromatography Technique and Its Use as Natural Antibiotic by Encapsulation Method
Diğer, ss.85-89, 2024
- Yayın Türü: Diğer Yayınlar / Diğer
- Basım Tarihi: 2024
- Sayfa Sayıları: ss.85-89
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Origanum species, which are widely used and marketed as spices, occupy a large place among plants collected from nature. The genus Origanum, belonging to the Labiatae family, consists of many species and subspecies with great interspecific and intraspecific diversity. More than half of the thyme exported from our country is obtained from cultivated thyme culture production. The thyme species Origanum onites and Origanum vulgare subsp. are grown around Isparta, Denizli and Izmir. Among the species traded in Turkey, Origanum onites L. is the most collected and exported species. The balls of Origanum onites L., which form the leaf and flower collection, are consumed as spices. The essential oil of Origanum onites L., which contains significant amounts of carvacrol and thymol, has antibacterial, antispasmodic, antiseptic, antimicrobial, cytotoxic, antioxidant and antifungal activity and the source of the therapeutic effect is shown to be carvacrol. The essential oil of the plant is used in food as well as in medicine and perfumery. In this study, essential oil was obtained from Origanum onites plant by distillation of water vapor. The essential oil was analyzed by GC/MSD instrument. According to the results, the main component carvacrol was determined as 73.11%. In this study, high purity carvacrol should be used. So, purification of the naturally occurring carvacrol compound in thyme was carried out using flash chromatography method and obtained %97,48 purity carvacrol. It is necessary to enable carvacrol to behave like the active ingredient of an antibiotic drug. Encapsulation method was considered as a method that can penetrate the cell wall and increase its effectiveness. In order to increase the effectiveness of this compound, the Liposome Encapsulation method was used, which can protect the molecule and facilitate its passage through the cell wall.