Lakes Region is an important area in cucurbit production of Turkey. During surveys conducted in the 20112012 growing season, field-grown squash plants with symptoms of mosaic, chlorotic mottling, vein banding, blistering and leaf malformation were observed. A total of 268 squash samples were collected from various locations in this region. Cucumber mosaic virus (CMV), was detected in squash, using enzyme-linked immunosorbent assay (ELISA), double stranded RNA (dsRNA) analysis and Reverse transcription polymerase chain reaction (RT-PCR) methods. ELISA tests showed that among 268 field samples tested, 54 were infected with CMV. ELISA-negative samples were tested by RT-PCR for CMV. All samples were positive for CMV. As a result of dsRNA analysis, specific 3 CMV RNA profiles were obtained.