IDENTIFICATION OF CUCUMBER MOSAIC VIRUS (CMV) ON SQUASH (CUCURBITA PEPO L.) CULTIVARS IN LAKES REGION OF TURKEY
FRESENIUS ENVIRONMENTAL BULLETIN, cilt.24, sa.2, ss.417-421, 2015 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 24 Sayı: 2
- Basım Tarihi: 2015
- Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.417-421
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Lakes Region is an important area in cucurbit production of Turkey. During surveys conducted in the 20112012 growing season, field-grown squash plants with symptoms of mosaic, chlorotic mottling, vein banding, blistering and leaf malformation were observed. A total of 268 squash samples were collected from various locations in this region. Cucumber mosaic virus (CMV), was detected in squash, using enzyme-linked immunosorbent assay (ELISA), double stranded RNA (dsRNA) analysis and Reverse transcription polymerase chain reaction (RT-PCR) methods. ELISA tests showed that among 268 field samples tested, 54 were infected with CMV. ELISA-negative samples were tested by RT-PCR for CMV. All samples were positive for CMV. As a result of dsRNA analysis, specific 3 CMV RNA profiles were obtained.