Design and synthesis of aminothiazole-benzazole based amide: antiproliferative, antimigration activity and molecular docking studies

Creative Commons License

Arslan Karahan G., Erzurumlu Y., Muhammed M. T., Özçelik A. B.

Medicinal Chemistry Research, vol.34, no.2, pp.406-422, 2025 (SCI-Expanded, Scopus)

  • Publication Type: Article / Article
  • Volume: 34 Issue: 2
  • Publication Date: 2025
  • Doi Number: 10.1007/s00044-024-03344-7
  • Journal Name: Medicinal Chemistry Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Veterinary Science Database
  • Page Numbers: pp.406-422
  • Keywords: 1,3-benzazol-2(3H)-one, 2-aminothiazole, Amide, Antimigration, Antiproliferative
  • Süleyman Demirel University Affiliated: Yes

Abstract

In this study, we synthesized a series of amide-functionalized aminothiazole-benzazole analogs for potential application in cancer treatment. The chemical structures of these compounds were confirmed using proton nuclear magnetic resonance (1H NMR), carbon-13 nuclear magnetic resonance (13C NMR) spectroscopy, and high-resolution mass spectrometry (HRMS). We evaluated the cytotoxicity of these compounds against breast cancer cells (MCF-7) and lung adenocarcinoma cells (A549). Notably, Compound 6b demonstrated significant cytotoxicity, with IC50 values of 17.2 ± 1.9 μM for MCF-7 cells and 19.0 ± 3.2 μM for A549 cells. Furthermore, we assessed the antimigration properties of all synthesized compounds, observing promising antiproliferative effects in both MCF-7 and A549 cells. Compound 6b exhibited a significant antimigration effect, achieving a 50.2 ± 4.7% wound healing rate in MCF-7 cells. In addition, we examined the impact of these compounds on key apoptotic proteins, including Caspase-7, PARP-1, BAX, and Bcl-2, which are critical in the regulation of programmed cell death. The binding potentials of the active compounds to BAX and Bcl-2 were also supported by docking. Results that consolidate the in vitro study were obtained from the in silico analysis. Our results suggest that these amide-functionalized aminothiazole-benzazole analogs exhibit potential as anticancer agents and merit further investigation to elucidate their mechanisms of action and therapeutic potential. Graphical abstract: (Figure presented.)