Leaves and flowers of Ajuga chamaepitys subsp. chia var. chia and Ajuga orientalis, which were taken from both their natural habitat and cultivars, were analyzed by SPME and GC-MS. The amount and the composition of volatile oil of cultivated plants were compared with natural area plants. beta-pinene was detected as major component in A. chamaepitys in both natural habitat and cultivars. It was seen that its amount was between 17.86-25.21%. Moreover, germacrene-D was determined in significant amount (15.40-21.13%) in both samples of A. chamaepitys. 2-hexen-l-al was the most abundant component in the natural area (30.89%) and cultivated plants (23.12%) in the first vegetation period of A. orientalis but a decrease of almost 80% was determined in the second vegetation period. The amount of 1-octen-3-ol was found as one of the dominant components in A. orientalis and no significant difference was observed in both natural habitat and cultivars, during the vegetation periods. In addition, the amount of limonene in A. orientalis volatile oil increased in the second vegetation period compared to the first.