Complex Analysis of Fluorescence Intensity Fluctuations of Molecular Compounds
JOURNAL OF APPLIED SPECTROSCOPY, cilt.87, sa.4, ss.685-692, 2020 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 87 Sayı: 4
- Basım Tarihi: 2020
- Doi Numarası: 10.1007/s10812-020-01055-6
- Dergi Adı: JOURNAL OF APPLIED SPECTROSCOPY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, INSPEC
- Sayfa Sayıları: ss.685-692
- Anahtar Kelimeler: fluorescence intensity fluctuation, photon counting histogram, molecular compounds, protein oligomers, data mining, principal component analysis, hierarchical cluster analysis, green-fluorescent protein (GFP)
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Süleyman Demirel Üniversitesi Adresli: Hayır
Özet
A method is proposed for the complex analysis of fluctuations in the fluorescence intensity of molecular compounds, which allows determining the structural composition of protein oligomers. The idea of the method is to analyze the photon counting histograms of experimental measurements using principal component analysis to assess the presence of oligomeric compounds, and to perform hierarchical cluster analysis, to determine the data classes corresponding to various molecular compounds, followed by selecting cluster medoids to determine the oligomeric composition of protein complexes. The efficiency of the analysis algorithms developed within the framework of the proposed method was confirmed on simulated and experimental photon counting histograms of the measured fluorescence intensity fluctuations of monomeric and dimeric forms of green-fluorescent protein (GFP).