Use of green RPLC methods as an alternative to conventional RPLC methods in the determination of the pKa values of some H2 receptor antagonists
Journal of Liquid Chromatography and Related Technologies, 2024 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 2024
- Doi Numarası: 10.1080/10826076.2024.2428185
- Dergi Adı: Journal of Liquid Chromatography and Related Technologies
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
- Anahtar Kelimeler: Binary mixture, green chemistry, H2 receptor antagonists, hybrid micellar chromatography, pKa value
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
In this study, the chromatographic retention behavior and dissociation constants (pKa) of H2 receptor antagonists ranitidine and famotidine were determined by the green reverse phase liquid chromatography (RPLC) and hybrid micellar chromatography methods, which are widely used today. Replicate analyses were performed on a Gemini NX C18 (250 × 3.0 mm ID, 5 μm) column at constant temperature (37 °C) and flow rate (1 mL/min). The obtained data analyses were evaluated with the linear solvation energy relationship (LSER) approach and the consistency of the data was assessed by calculating the theoretical retention time values. The (Formula presented.) and (Formula presented.) values obtained in ethanol–water binary mixtures containing 11%, 13%, 15%, and 20% (v/v) ethanol were also calculated with this approach. The pKa values of these compounds in water and pure micellar environment were calculated with (Formula presented.) values and solvent. It was observed that the (Formula presented.) data obtained by three different methods were compatible. Accordingly, with the hybrid micelles method, (Formula presented.) and (Formula presented.) values for famotidine were calculated as 7.305 and 9.869, respectively, and (Formula presented.) and (Formula presented.) values for ranitidine were calculated as 2.952 and 8.589. The environmental impacts of the methods applied in the study were evaluated using green metric tools.