Aim: The increase in the usage of radiologic imaging methods today increases the risk of medical staff to be exposed to more radiation. In our study, the radiation safety awareness of medical staff and factors affecting this awareness were examined. Material and Method: In this study planned as descriptive, the medical staffs, who work in nuclear medicine, radiology and radiation oncology departments at four different centers, were asked survey questions prepared by researchers. Results: A total of 184 medical staff was involved in the study from 4 different centers. There was significant difference determined regarding the usage and safety of lead vest related to the duties of attendants (p<0.001, p:0.013). There was a significant relation determined between dosimeter usage and the duties of attendants (p:0.006). There were a significant relation between the duties of attendants and radiation training (p<0.001). Discussion: In order for the medical staff exposed to radiation to give more quality service, the staff should learn how to protect from radiation. Radiation safety committees should work actively, give radiation training to the staff, and provide that doctors also participate in these trainings.