In recent times, pomegranate has been one of Turkey's most important commercial fruit crops for consumption and export. In this study, the chemical composition of pomegranate (Punica granatum L.) fruits grown in the central area of Bitlis province (Eastern Turkey) was investigated. For this purpose, total phenolic content, ascorbic acid content, total anthocyanin and antioxidant activity and minerals content were evaluated. The highest total phenolic contents were determined in 13BIT1 (6477.78 mg gallic acid equivalents 100 g(-1) fresh matter). The highest ascorbic acid was determined in 13BIT2 of pomegranate genotype (60.78 mg 100 g(-1)). Radical scavenging activity (DPPH) were determined between 13BIT18 (78.15) to 13BIT1 (31.49). Total anthocyanin of genotypes was measured between 13BIT19 (156.03) to 13 BIT17 (55.37), respectively. The highest mineral compositions of the pomegranate genotypes were 998.00% N, 301.00 mg 100 g(-1) P, 1708.61 mg 100 g(-1) K, 55.21 mg 100 g(-1) Ca, 116.79 mg 100 g(-1) Mg, 5.1 mg 100 g(-1) Fe, 1.91 mg 100 g(-1) Cu, 0.41 mg 100 g(-1) Mn and 1.20 mg 100 g(-1) Zn, respectively. The results indicate that pomegranate genotypes have an important value of health and nutrition for the human.