Apoptotic Effect of Quercetin on Human Colon Cancer Cell Line SW-480
7.Oksidatif Stres, Kalsiyum Sinyali ve TRP Kanalları Dünya Kongresi, Antalya, Türkiye, 20 - 23 Nisan 2018, ss.691-692, (Özet Bildiri)
- Yayın Türü: Bildiri / Özet Bildiri
- Basıldığı Şehir: Antalya
- Basıldığı Ülke: Türkiye
- Sayfa Sayıları: ss.691-692
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Cancer is the second cause of death and colon
cancer is the most prevalent cancer worldwide so
treatment alternatives are always on the agenda. The
etiology of human colon cancer is very complex, but
diet is considered to be one of the important reason of it
and researches think that lots of people are at risk of
colon cancer because diet style. In light of this
information, we aimed to investigate whether Quercetin
(Que) has apoptotic effect on SW-480 colon cancer
cells.
Quercetin is a type of bioflavonoid naturally
occurring in various edible plants. Also it has antiproliferative effects. The SW-480 colon cancer cell line
was cultured in monolayer model. Cells were treated
with Que at 24, 48 and 72 hours of incubation. TUNEL
assay were used to determine the apoptotic cells in the
monolayer culture. The BrdU labeling index was used to determine the proliferation of cells.
An IC 50 inhibition dose of Que in SW-480 colon
cancer cell was 200 µM/ml at 24, 48 and 72 hours of
incubation. The control group had a normal pattern of Sphase fraction and many of the SW-480 cells nuclei
were observed to be positive for BrdU. TUNEL positive
cells were detected after treatment with Que in the
monolayer cultures. The dead cell count was higher in
the SW-480 cell lines with Que applied than in the
control.
We concluded that Que inhibit colon cancer
growth by apoptosis with use caspase pathway. Further
in vivo studies are needed to confirm our findings in
humans. Three-dimensional studies, caspase analyses
and AIF immunestaining analyses are still ongoing.