This study was conducted to examine the effect of soil, leaf and soil+leaf applications of humic substance on fruit yield, some quality parameters and mineral nutrition of Granny Smith and Jersey Mac apple varieties grafted on MM106 rootstock. The study was carried out for two consecutive years. According to the results obtained from both years, humic substance applications had no significant effect on fruit yield and quality parameters generally, but relative increases were recorded in yields. Leaf N, K, Ca, Fe and Zn concentrations were significantly affected from the applications. At the first year humic substance application significantly affected only N and K concentrations of Jersey Mac variety, but in the second year, humic substance applications led to increase in leaf N, K, Ca, Fe and Zn concentrations in Granny Smith and N concentration in Jersey Mac apple variety. According to the results obtained, it can be said that the effects of humic substances were higher than the first year's effects mostly.