This research was aimed to determine the effects of plant regulators such as gibberellic acid (GA(3)), aminoethoxyvinylglycine (AVG), and choline chloride (CC) on the nutrient components in the fruit of Monroe' peach under Isparta in Turkey ecological condition. AVG at doses of 0, 100, 150 and 200ppm, GA(3) at doses of 0, 100, 200, 300ppm and CC at doses of 0, 1000, 2000, 3000pmm was sprayed on the peaches 7, 21 and 30days before commercial harvests. All concentrations of AVG treatments were found to increase the amount of nitrogen in fruits. In general, it was observed that CC and GA(3) applications reduced the amount of calcium in fruits, however, AVG applications were found to not be effective. In the first year, the statistically significant of AVG treatments was seen on magnesium content only. It was determined in our study that AVG and CC treatments reduced copper content in peach fruits, all applications decreased the amount of manganese, 100ppm AVG application increased the element zinc, and all of CC applications minimized the amount of that element.