The aim of this study is to determine the germination ability and biochemical properties of seedlings of two taxa (Ajuga chamaepitys subsp. chia var. chia, Ajuga orientalis) belonging to the Lamiaceae family which is naturally distributed in Lakes District-Turkey and cultivated in the Botanic Garden of Suleyman Demirel University in Isparta. These two taxa, which have usage potential as ornamental, medicinal and aromatic plants, were cultivated in 2015 vegetation period and seeds were collected at the end of 2016 vegetation period. During the same vegetation period, seeds were also collected from the areas where these two species were naturally grown. Seed germination tests were performed in 2017 vegetation period on the plants. Seed germination tests and seedling raising tests were carried out in the seedling and phenological properties (plant height, plant diameter, leaf width, leaf length) and SPAR values were also measured. Some macro and micro elements (Total N, P, K, Ca, Mg, Fe, Cu, Mn and Zn) and volatile oil compounds were analysed in the samples taken. As a result of the study, germination was not observed in Ajuga chamaepitys subsp. chia var. chia; however, seedling raising rate was between 33% and 40%. Seedling raising speed was 33.7% in the trial area and 36.41% in the seeds collected from the natural habitat. The germination rate of Ajuga orientalis was found to be 18% in the seeds taken from the natural habitat and 21.33% in the seeds taken from cultivars. Seedling raising rate was 43.33% in the seeds taken from the natural habitat and 60% in the seeds obtained from the trial area. Although the germination ability of the seeds from both origins (natural habitat and cultivars) were low, the phenological properties measurements and the biochemical analysis results were in a close relation with each samples.