Fluorescence study of protein immobilization on poly(4-hydroxyphenyl thiophene-3-carboxylate)-coated electrodes

Uygun, Ayşegül; ÖKSÜZ, Lütfi; Chowdhury, Sanchari; Bhethanabotla, Venkat

doi:10.1016/j.msec.2010.03.025

Fluorescence study of protein immobilization on poly(4-hydroxyphenyl thiophene-3-carboxylate)-coated electrodes

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Uygun A., ÖKSÜZ L., Chowdhury S., Bhethanabotla V.

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, vol.30, no.6, pp.868-872, 2010 (SCI-Expanded)

Publication Type: Article / Article
Volume: 30 Issue: 6
Publication Date: 2010
Doi Number: 10.1016/j.msec.2010.03.025
Journal Name: MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Page Numbers: pp.868-872
Süleyman Demirel University Affiliated: Yes

Abstract

Synthesis of poly(4-hydroxyphenyl thiophene-3-carboxylate) (PHPT) was carried out in acetonitrile(ACN)/TBAClO(4)(0.05 M) solvent-electrolyte medium using different working electrodes (glassy carbon and ITO) via potentiodynamic electrolysis. Characterization of the resulting polymer was performed using cyclic voltammetry (CV), scanning electron microscopy (SEM), atomic force microscopy (AFM) and fluorescence microscopy (FS). The polymer exhibits well-defined and reversible redox behavior in an organic solvent. Flourescence microscopy indicates that protein immobilizes better onto the surface of PHPT-modified electrodes than onto those without such modification. (C) 2010 Elsevier B.V. All rights reserved.