This study was conducted to determine of some chemical properties of 'Sweet Ann' and 'Kabarla' strawberry cultivars in highland climate. This research was carried out in Isparta region with highland climate conditions. Both varieties of strawberry cultivars used in the research are neutral day plants. Due to the rich polyphenol content of the berries, the positive effects on human health have begun to be explored in recent years. Especially high anthocyanin contents are important. It is well known that the strawberries are rich in polyphenol compounds. In this research, two different production methods were applied, open field and cover cultivation. The total phenolic, total anthocyanin and ascorbic acid content were determined. Total phenolic of 'Sweet Ann' strawberry cultivar varied between 620.44mg GAE 100 g(-1) FW to 786.64 mg GAE 100 g(-1) FW. Total anthocyanin of 'Sweet Ann' strawberry cultivar and ascorbic acid ranged between 58.15 mu g/g to 98.88 mu g/g and 76.42 mg/100 g to 94.39 mg/100 g respectively. Total phenolic of 'Kabarla' strawberry cultivar varied between 448.01 mg GAE 100 g(-1) FW to 1050.48 mg GAE 100 g-1 FW. Also total anthocyanin of 'Kabarla' strawberry cultivar ranged between 47.16 mu g/g to 74.44 mu g/g. Ascorbic acid content was determined by 125.09 mg/100 g to 134.81 mg/100 g in 'Kabarla' strawberry cultivar. Highest value of total anthocyanin and ascorbic acid were obtained from open field cultivation for both varieties.