Nanofibers have the big potential to create absolutely new industries and application areas. In this study we demonstrated the effect of supporting material type on the fiber morphology such as diameter, diameter distribution and non-fibrous area. Poly (vinyl alcohol) (PVA), molecular weight of 88.000 g/mol was used as a polymer. Different supporting materials like polypropylen nonwoven antistatic material, black paper, various woven and knitting fabrics, carbon weaving and aluminum foil surface were used to collect the nanofibers. Nanofibrous materials were obtained using roller electrospinning method which has been known as Nanospider trade name. This method is one of the effective methods to produce nanofibers at industrial scale which was invented by Jirsak et. al. from the Technical University of Liberec. The same process parameters (solution concentration, voltage, distance between the electrodes, production time) were applied during the spinning process for all supporting materials. Then fiber morphology was analyzed using scanning electron microscope (SEM) and fiber diameter, diameter uniformity and non-fibrous area values were calculated. The best supporting material was determined after analyzed the effect of supporting material type on the fiber morphology.