Research Information System
International Journal of Scientific and Technological Research, vol.5, no.4, pp.20-26, 2019 (Peer-Reviewed Journal)
In this study, the
simultaneous determination of Lisinopril (LIS) and
Hydrochlorothiazide(HCT) in drug samples by chemometric approaches using
UV spectrophotometry has been reported as a simple alternative to using
separate models for each component. Spectra of LIS and
HCT were recorded at several concentrations within their ranges and were
used to compute the calibration mixture between wavelengths 200 and 350 nm at
an interval of 5 nm was used for data acquisition. Principal component
regression was used for chemometric analysis of data and the parameters of the
chemometric procedures were optimized. The analytical performances of this
chemometric method was characterized by predicted residual error sum of squares
(PRESS), standard error of prediction (SEP) and recoveries (%) and were
compared with each other. A series of synthetic solution containing different
concentrations of LIS and HCT were used to check the prediction ability of the
principal component regression. This method was successfully applied to real
samples, with no interference from excipients as indicated by recovery study
results. The outcomes acquired in this examination firmly urge us to apply
these strategies for a standard investigation and quality control of the two
medications.