To examine the effects of the silibinin on human breast cancer MDA -MB- 231 Cells


Bayram D. , Sakallı Çetin E., Kara M., Özgöçmen M. , Candan İ. A.

5th International Congress on Cell Membranes and Oxidative Stress: Focus on Calcium Signaling nad TRP Chanels, Isparta, Turkey, 9 - 12 September 2014, pp.407-408

  • Publication Type: Conference Paper / Summary Text
  • City: Isparta
  • Country: Turkey
  • Page Numbers: pp.407-408

Abstract

Aim Silibinin, is a bioactive flavonolignan extracted from milk thistle, Silybum marianum. Silibinin exerts strong anti-proliferative, pro-apoptotic and anti-inflammatory effects. Many studies have shown that silibinin inhibits experimentally induced malignancies of the liver, prostate, skin, and colon as well as inhibition of proliferation of cancer cell lines in vitro. This work aimed to study the effects of the Silibinin on human breast cancer cell line MDAMB-231 using Poly-ADP-Ribose-Polimerase (PARP) staining and Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Materials and Methods MDA-MB-231 cell line was cultured in monolayer model. Cells were treated with the Silibinin on 24, 48 and 72 hours incubation. Cells were not treated with the Silibinin were considered as the control group. The effects of the Silibinin effective dose on PARP and Tunel staining were assessed by immunohistochemically. Results IC50 values of TQ in MDA-MB-231 is 100µM, 50µM and 50µM on 24, 48 and 72 hours incubation respectively. PARP staining and Tunel assay was used together to determine the death of the cells. TUNEL positive cells and active PARP were detected after treatment in monolayer model. Dead cell count was more in Silibinin applied MDA-MB-231 cell lines in comparison to the controls (p <0.05). Conclusion In this study, Silibinin applications enhanced the TUNEL positive cells and active PARP in comparison to the controls in monolayer model.