5th International Congress on Cell Membranes and Oxidative Stress: Focus on Calcium Signaling nad TRP Chanels, Isparta, Turkey, 9 - 12 September 2014, pp.407-408
Silibinin, is a bioactive flavonolignan extracted
from milk thistle, Silybum marianum. Silibinin
exerts strong anti-proliferative, pro-apoptotic
and anti-inflammatory effects. Many studies have
shown that silibinin inhibits experimentally induced
malignancies of the liver, prostate, skin, and colon
as well as inhibition of proliferation of cancer cell
lines in vitro.
This work aimed to study the effects of the
Silibinin on human breast cancer cell line MDAMB-231 using Poly-ADP-Ribose-Polimerase (PARP)
staining and Terminal deoxynucleotidyl transferase
dUTP nick end labeling (TUNEL) assay.
Materials and Methods
MDA-MB-231 cell line was cultured in monolayer
model. Cells were treated with the Silibinin on 24,
48 and 72 hours incubation. Cells were not treated
with the Silibinin were considered as the control
group. The effects of the Silibinin effective dose
on PARP and Tunel staining were assessed by
IC50 values of TQ in MDA-MB-231 is 100µM,
50µM and 50µM on 24, 48 and 72 hours incubation
respectively. PARP staining and Tunel assay was used together to determine the death of the cells.
TUNEL positive cells and active PARP were detected
after treatment in monolayer model. Dead cell count
was more in Silibinin applied MDA-MB-231 cell lines
in comparison to the controls (p <0.05).
In this study, Silibinin applications enhanced the
TUNEL positive cells and active PARP in comparison
to the controls in monolayer model.