To Examine the effects of the thymoquinone on human breast cancer MDA-MB-231 cells


Sakallı Çetin E., Bayram D. , Kara M., Candan İ. A. , Özgöçmen M.

5th International Congress on Cell Membranes and Oxidative Stress:Focus on Calcium Signaling and TRP Channels, Isparta, Turkey, 9 - 12 September 2014, vol.6, pp.407

  • Publication Type: Conference Paper / Summary Text
  • Volume: 6
  • City: Isparta
  • Country: Turkey
  • Page Numbers: pp.407

Abstract

Aim Thymoquinone (TQ), phytochemical compound extracted from the plant Nigella sativa or black cumin, exhibits medicinal effects, including antibacterial, anti-fungal, anti-viral, anti-inflammatory, immunomodulatory, and anti-cancer properties. Furthermore, TQ inhibits human cancer-cell proliferation and induces apoptosis. This work aimed to study the effects of the on human breast cancer cell line MDA-MB-231 using Poly-ADP-Ribose-Polimerase (PARP) staining and Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Materials and Methods MDA-MB-231 cell line was cultured in monolayer model. Cells were treated with the TQ on 24, 48 and 72 hours incubation. Cells were not treated with the TQ were considered as the control group. The effects of the TQ effective doses on PARP and Tunel staining were assessed by immunohistochemically. Results IC50 values of TQ in MDA-MB-231 are 200µM on 24, 48 and 72 hours incubation. PARP staining and Tunel assay was used together to determine the death of the cells. TUNEL positive cells and active PARP were detected after treatment in monolayer model. Dead cell count was more in TQ applied MDA-MB-231 cell lines in comparison to the controls (p <0.05). Conclusion In this study, Nigella sativa applications enhanced the TUNEL positive cells and active PARP in comparison to the controls in monolayer model