5th International Congress on Cell Membranes and Oxidative Stress:Focus on Calcium Signaling and TRP Channels, Isparta, Turkey, 9 - 12 September 2014, vol.6, pp.407
Thymoquinone (TQ), phytochemical compound
extracted from the plant Nigella sativa or black
cumin, exhibits medicinal effects, including antibacterial, anti-fungal, anti-viral, anti-inflammatory,
immunomodulatory, and anti-cancer properties.
Furthermore, TQ inhibits human cancer-cell
proliferation and induces apoptosis.
This work aimed to study the effects of the on
human breast cancer cell line MDA-MB-231 using
Poly-ADP-Ribose-Polimerase (PARP) staining and
Terminal deoxynucleotidyl transferase dUTP nick
end labeling (TUNEL) assay.
Materials and Methods
MDA-MB-231 cell line was cultured in monolayer
model. Cells were treated with the TQ on 24, 48 and
72 hours incubation. Cells were not treated with
the TQ were considered as the control group. The
effects of the TQ effective doses on PARP and Tunel
staining were assessed by immunohistochemically.
IC50 values of TQ in MDA-MB-231 are 200µM on
24, 48 and 72 hours incubation. PARP staining and
Tunel assay was used together to determine the
death of the cells. TUNEL positive cells and active
PARP were detected after treatment in monolayer
model. Dead cell count was more in TQ applied
MDA-MB-231 cell lines in comparison to the controls
In this study, Nigella sativa applications
enhanced the TUNEL positive cells and active PARP
in comparison to the controls in monolayer model