The Role of Flupyradifuron in Inducing Apoptosis in HMC3 Cells: An In Vitro Analysis
Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi, cilt.33, sa.1, ss.79-85, 2026 (TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 33 Sayı: 1
- Basım Tarihi: 2026
- Doi Numarası: 10.17343/sdutfd.1839335
- Dergi Adı: Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi
- Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.79-85
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Objective Flupyradifurone, a neonicotinoid insecticide, is widely applied in pest management. The present study aimed to elucidate the underlying mechanisms of flupyradifurone-induced toxicity in HMC3 microglial cells. Material and Method Cell viability was assessed by the MTT assay following exposure to flupyradifurone. In parallel, the expression of key apoptotic pathway genes, including MDM2, p53, and CASP9, as well as the apoptosisand cell viability–associated microRNA hsa-miR-21-5p, was evaluated using RT-PCR. Results The results demonstrated that flupyradifurone induced dose-dependent cell death in HMC3 cells. RT-PCR analysis revealed a significant downregulation of MDM2, miR-21-5p, and an upregulation of p53 and CASP9 after 24 h of treatment. Conclusion This study reveals that FDP causes cell death in HMC3 cells, and this process occurs through apoptosis.