Presence of Lactobacillus kefiranofaciens subsp. kefiranofaciens, Lentilactobacillus kefiri and Lentilactobacillus parakefiri in the stools of Balb/c consuming natural kefir

KÖK TAŞ T., Atilgan S., ÖZDEMİR N.

BIOLOGIA, vol.77, no.8, pp.2393-2403, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 77 Issue: 8
  • Publication Date: 2022
  • Doi Number: 10.1007/s11756-022-01094-8
  • Journal Name: BIOLOGIA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Animal Behavior Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Veterinary Science Database
  • Page Numbers: pp.2393-2403
  • Keywords: Lactobacillus kefiranofaciens subsp, kefiranofaciens, Lentilactobacillus parakefiri, Lentilactobacillus kefiri, In vivo evaluation, Kefir-grain, GUT MICROBIOTA, MOUSE MODEL, MICE, IDENTIFICATION, MODULATION
  • Süleyman Demirel University Affiliated: Yes


This study was aimed to determine how much probiotic-LAB strains belonging to L. kefiranofaciens, L. parakefiri and L. kefiri species species found in natural kefir-grain were transferred to the feces of experimental-animals and how long they colonized to intestinal-systems of experimental animals. A total of 30 male Balb/c mice were fed with the kefir-drink produced using kefir-grain. Feces samples, collected during 15 days, were analyzed by qPCR. As a result, it was determined that the transfer of the L. kefiranofaciens, the L. kefiri, and especially the L. parakefiri strains, which specific to natural-kefir, produced using kefir-grain, into mice feces microbiota was determined for the first time. In the intestinal-microbiota of the mice, the L. kefiranofaciens strain had higher colonization efficiencies than others. Besides, the L. parakefiri strain with the highest amount of increase supported the mice-feces-microbiota. Thus, the efficacy of natural-kefir in transferring tested probiotic strains to the mice-feces was proven.