Preventive Effect of Probiotics and alpha-Tocopherol on Ethanol-Induced Gastric Mucosal Injury in Rats

ŞENOL A. , ISLER M., KARAHAN A. G. , Kilic G. B. , KULEAŞAN H. , KAYA S. , ...More

JOURNAL OF MEDICINAL FOOD, vol.14, pp.173-179, 2011 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 14
  • Publication Date: 2011
  • Doi Number: 10.1089/jmf.2010.0040
  • Title of Journal : JOURNAL OF MEDICINAL FOOD
  • Page Numbers: pp.173-179


The protective effect of a probiotic mixture of 13 different bacteria and alpha-tocopherol on 98% ethanol-induced gastric mucosal injury was evaluated. Levels of gastric mucosal pro- and anti-inflammatory cytokines, malondialdehyde, and secretory immunglobulin A were measured. Rats were allocated into four groups: control, ethanol, probiotic, and alpha-tocopherol. The control and ethanol groups received skim milk for 14 days. Probiotic and alpha-tocopherol groups were administered probiotic mixture suspended in skim milk and 100 mg/kg alpha-tocopherol, respectively, by daily gavage for 14 days. On Day 15, gastric lesions were induced by administration of ethanol 98% (1 mL) to all rats except those in the control group. Probiotic, but not alpha-tocopherol, seemed to inhibit ethanol-induced gastric mucosal tumor necrosis factor-a, interferon-g, and interleukin-2 production (P>.05). Ethanol caused the elevation of mucosal interleukin-4 level (compared to the control, P<.05). Probiotic pretreatment significantly suppressed the ethanol-induced increase of gastric mucosal interleukin-4 levels. Pretreatment with either probiotic or alpha-tocopherol inhibited the ethanol-induced increase of mucosal malondialdehyde concentration (P<.01 and P<.05, respectively). Probiotic pretreatment enhanced the gastric mucosal secretory immunoglobulin A concentration (P<.001). In conclusion, probiotic mixture and alpha-tocopherol reduced ethanol-induced gastric mucosal lipid peroxidation, suggesting that they may be beneficial for gastric lesions induced by lower ethanol concentration.