Preventive Effect of Probiotics and alpha-Tocopherol on Ethanol-Induced Gastric Mucosal Injury in Rats
JOURNAL OF MEDICINAL FOOD, cilt.14, ss.173-179, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 14
- Basım Tarihi: 2011
- Doi Numarası: 10.1089/jmf.2010.0040
- Dergi Adı: JOURNAL OF MEDICINAL FOOD
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.173-179
- Anahtar Kelimeler: ethanol, gastric injury, probiotic, rat, alpha-tocopherol, LACTOBACILLUS-RHAMNOSUS GG, PALM VITAMIN-E, FREE-RADICALS, ESCHERICHIA-COLI, ISCHEMIA-REPERFUSION, ULCERATIVE-COLITIS, DAMAGE, INFLAMMATION, VSLNUMBER-3, SUPEROXIDE
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
The protective effect of a probiotic mixture of 13 different bacteria and alpha-tocopherol on 98% ethanol-induced gastric mucosal injury was evaluated. Levels of gastric mucosal pro- and anti-inflammatory cytokines, malondialdehyde, and secretory immunglobulin A were measured. Rats were allocated into four groups: control, ethanol, probiotic, and alpha-tocopherol. The control and ethanol groups received skim milk for 14 days. Probiotic and alpha-tocopherol groups were administered probiotic mixture suspended in skim milk and 100 mg/kg alpha-tocopherol, respectively, by daily gavage for 14 days. On Day 15, gastric lesions were induced by administration of ethanol 98% (1 mL) to all rats except those in the control group. Probiotic, but not alpha-tocopherol, seemed to inhibit ethanol-induced gastric mucosal tumor necrosis factor-a, interferon-g, and interleukin-2 production (P>.05). Ethanol caused the elevation of mucosal interleukin-4 level (compared to the control, P<.05). Probiotic pretreatment significantly suppressed the ethanol-induced increase of gastric mucosal interleukin-4 levels. Pretreatment with either probiotic or alpha-tocopherol inhibited the ethanol-induced increase of mucosal malondialdehyde concentration (P<.01 and P<.05, respectively). Probiotic pretreatment enhanced the gastric mucosal secretory immunoglobulin A concentration (P<.001). In conclusion, probiotic mixture and alpha-tocopherol reduced ethanol-induced gastric mucosal lipid peroxidation, suggesting that they may be beneficial for gastric lesions induced by lower ethanol concentration.