Transfer of powdery mildew resistance from Brassica carinata to Brassica oleracea through embryo rescue


Tonguc M. , GRIFFITHS P.

PLANT BREEDING, cilt.123, ss.587-589, 2004 (SCI İndekslerine Giren Dergi) identifier identifier

Özet

Interspecific hybrid plants and backcross 1 (BC1) progeny were produced through sexual crosses and embryo rescue between Brassica carinata accession PI 360883 and B. oleracea cvs 'Titleist' and 'Cecile' to transfer resistance to powdery mildew to B. oleracea. Four interspecific hybrids were obtained through application of embryo rescue from crosses with B. carinata as the maternal parent, and their interspecific nature confirmed through plant morphology and random amplified polymorphic DNA (RAPD) analysis. Twenty-one BC1 plants were obtained through sexual crosses and embryo rescue although embryo rescue was not necessary to produce first backcross generation plants between interspecific hybrids and B. oleracea. All interspecific hybrids and eight of the BC1 plants were resistant to powdery mildew.