Natural health products and functional foods are
frequently consumed for the prevention and treatment
of many diseases. Flaxseed is one of the functional foods with anticancer, antiviral, antibacterial and antifungal properties. In addition to its beneficial effects,
excessive consumption without considering the appropriate dosage can cause toxic effects. In this study,
it was planned to investigate the effects of different
amounts of flaxseed consumption on rat liver.
Materials and Methods
Rats were randomly divided into four groups with 8
rats in each groups; control group and experimental
groups which given flaxseed for 7 days; 1.4, 2.8 and
5.6 g/kg/day, respectively. At the end of the experiment, histological, immunohistochemical and biochemical analyzes were performed on tissue and blood
Serum AST, ALT and ALP values are significantly higher in 5.6 g/kg of flaxseed compared to control group
(p values 0.003, 0.012 and 0.009, respectively). Albumin values were significantly lower in experimental
groups compared to the control group (p<0.05). TOS
and OSI increased significantly (p<0.05), TAS decreased (p>0.05) in all experimental groups compared to
control. In histopathological findings, there was significant difference in three groups compared to control
group, especially in 5.6 g/kg of flaxseed group compared to others. As a result of immunohistochemical
analyzes, staining intensity of the receptors was highest in 5.6 g/kg of flaxseed group. The highest positive staining was observed respectively in NOX4
TNF-α and IL-6.
These results show that excessive consumption of
flaxseed can cause oxidative stress and toxicity due
to inflammation in the liver and this study can helpful
other studies about flaxseed toxicity.