Silymarin Ameliorates Valproic Acid-Induced Pancreas Injury by Decreasing Oxidative Stress

Aktaş İ., Gür F. M. , Özgöçmen M.

International Journal of Veterinary and Animal Research, vol.3, no.2, pp.34-38, 2020 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 3 Issue: 2
  • Publication Date: 2020
  • Journal Name: International Journal of Veterinary and Animal Research
  • Page Numbers: pp.34-38


In this study, the protective effects of silymarin (SLY) against valproic acid (VPA) induced pancreatic damage was investigated in rats by using histological and biochemical methods. The experiment was performed with 21 Sprague Dawley male rats. Rats were divided into three groups: The control group received 0.5 mL saline via gavage, VPA group received 500 mg/kg/day VPA by gavage and VPA+SLY group received 500 mg/kg/day VPA+ 100 mg/kg/day SLY via gavage for 14 days. Serum amylase and lipase levels, which increased as a result of VPA application, were reversed with SLY application. In addition, with VPA administration, malondialdehyde (MDA) increased and glutathione (GSH) levels decreased in pancreatic tissue. This situation was significantly (p ≤ 0.05) suppressed with the application of SLY. Histopathological changes such as acinar cell degeneration, bleeding, infiltration, necrosis and shrinkage in Langerhans islets were observed after VPA application. Reactive oxygen species (ROS) and histopathological changes decreased and antioxidant activity increased in VPA + SLY group compared to VPA group. The results obtained in this study revealed that SLY is a potential pharmacological agent that can be used as a protective and therapeutic agent against VPA-induced pancreatic damage.